Edvotek Code Breakers Experiment Kit: Using CRISPR to Rewrite Genetics

Unlock the exciting world of gene editing and investigate the incredible power of CRISPR technology right from your classroom! In this experiment, students explore cutting-edge genetic engineering using CRISPR-Cas9 to knock out the purple chromogenic genes in classroom safe bacteria. Additionally, students test the specificity of CRISPR-Cas9 first hand by using a non-specific CRISPR gRNA templates and analysing the results.

• Explore genetic engineering, CRISPR, and the central dogma of molecular biology in this comprehensive experiment
• Transform classroom-safe E. coli with CRISPR RNA templates to test the targeting specificity of the technology
• Identify successful gene knockout based on the colour of the cells
• Group size: for 10 lab groups
• Time required: set up - 50 minutes, incubation - overnight, transformation - 90 minutes
• Kit includes: instructions, LyphoCells™ E. coli Purple, ampicillin, IPTG, Chloramphenicol, ReadyPour™ SOC-agar, pCas9-PurpleGuide (plasmid), pCas9-ControlGuide (plasmid), CaCl₂, competent cell solution, SOC liquid medium (broth), SOC additive (for ReadyPour ONLY), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10mL pipet (sterile), inoculating loops (sterile), microcentrifuge tubes, and 50mL conical tubes
• All you need: automatic micropipette (5 to 50µL) and tips, two water baths (37°C and 42°C), incubation oven (optional but recommended), thermometer, centrifuge(s), vortexer (optional), floating racks, pipet pumps or bulbs, ice, marking pens, tape, hot plate or microwave oven, and hot gloves
• Storage: some components require freezer storage upon receipt